K610 Laboratory (EBI)

P. aeruginosa RcsA in response to bleach

Covid-19

The World Health Organization says the official name for the disease caused by the new coronavirus is Covid-19.

TtcA requires Fe-S for KatA fighting

Proposed model of OxyR-regulated katA and ttcA expression under oxidative stress conditions. P. aeruginosa OxyR upregulates katA and ttcA expression to increase catalase activities in response to H2O2 generated by host defence mechanisms. The four cysteine residues required for fully functional TtcA activity to have a role in the oxidative stress response via KatA activity and facilitates bacterial survival during infection.

Antibiotic resistance breakers

Several marketed drugs that do not currently have antibacterial indications can either directly kill bacteria, reduce the antibiotic minimum inhibitory concentration when used in combination with existing antibiotics and/or modulate host defence through effects on host innate immunity, in particular by altering inflammation and autophagy.

Periplamic Msr system

MsrPQ is essential for the maintenance of envelope integrity under bleach stress, rescuing a wide series of structurally unrelated periplasmic proteins from methionine oxidation, including the primary periplasmic chaperone SurA. For this activity, MsrPQ uses electrons from the respiratory chain, which represents a novel mechanism to import reducing equivalents into the bacterial cell envelope. A remarkable feature of MsrPQ is its capacity to reduce both R and S forms.

Defining bacterial regulons ChlP Seq

Dr. Patricia J. Kiley and her colleges, University of Wisconsin - Madison, have published this great article in Method journal. Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) is a powerful method that identifies protein-DNA binding sites in vivo.

Fosfomycin and carbapenems for PAE

Synergistic effects of fosfomycin and carbapenems against carbapenem-resistant Pseudomonas aeruginosa clinical isolates. Int J Antimicrob Agents. 2015 Feb 10. pii: S0924-8579(15)00053-9. doi: 10.1016/j.ijantimicag.2015.01.005. Kunakonvichaya B, Thirapanmethee K, Khuntayaporn P, Montakantikul P, Chomnawang MT. Department of Microbiology, Faculty of Pharmacy, Mahidol University

Antisense small RNAs in PAE

BMC Genomics. 2014 Sep 11;15:783. doi: 10.1186/1471-2164-15-783. Expression of antisense small RNAs in response to stress in Pseudomonas aeruginosa. Gómez-Lozano M, Marvig RL, Tulstrup MV, Molin S. In this study we used RNA-seq to identify 232 antisense RNAs (asRNAs) in the opportunistic pathogen Pseudomonas aeruginosa grown under 13 different conditions.

YfiR from PAE in two redox states

The crystal structures of YfiR from Pseudomonas aeruginosa were determined. YfiR exists as a dimer in both the crystal and solution. Disulfide bond C145–C152 plays an important role in maintaining the correct folding of YfiR. The Cys145-containing β5–β6 loop is flexible and close to the proposed YfiN binding sites.

CysB negatively affects pqsR in PAE

The production of PQS and other 4-hydroxy-2-alkylquinolone (HAQs) compounds is a key component of the P. aeruginosa cell-to-cell signaling network, impacts multiple physiological functions, and is required for virulence. PqsR directly regulates the genes necessary for HAQ production, but little is known about the regulation of pqsR. We identified CysB as a novel regulator of pqsR and PQS production. This implies that CysB functions as both a cysteine-responsive and unresponsive regulator.

Zinc induces phosphoethanolamine

Extracellular zinc induces phosphoethanolamine addition to Pseudomonas aeruginosa lipid A via the ColRS two-component system. Mol Microbiol. 2015 Apr 2. Nowicki EM, O'Brien JP, Brodbelt JS, Trent MS. Department of Molecular Biosciences, University of Georgia, College of Veterinary Medicine, Athens, Georgia, USA.